Trichoderma:A Plant Growth Promoting Fungi

Authors

Ashok Kumar, Research Associate
Department of Zoology, UCOS, MLSU, Udaipur, Rajasthan, India.

Divya Shrivastava, Professor and Director
School of Life Sciences, Jaipur National University, Jagatpura, Jaipur, Rajasthan, India.

Meenal Dixit, Assistant Professor
Jaipur National University, Jagatpura, Jaipur, Rajasthan, India.

Sanjay Kumar Meena, Research Scholar
Department of Zoology, UCOS, MLSU, Udaipur, Rajasthan, India.

Suman, Research Scholar
Department of Zoology, UCOS, MLSU, Udaipur, Rajasthan, India.

Arti Prasad, Professor and Head
Department of Zoology, UCOS, MLSU, Udaipur, Rajasthan, India.

Abstract

Plant growth promoting fungi improve plant growth, development and productivity in many crops. Trichoderma spp., a major cultivable PGPF in soils, extensively promotes plant growth biomass and yield by improving the creation of plant stimulatory compounds, such as growth hormones (Indole Acetic Acids, Cytokinin, Gibberellins and Zeatin) and by the secretion of antifungal substances i.e., iron chelators (Siderophore), hydrogen cyanide and Ammonia, which can protect plants from infection by phytopathogens and promotes plant growth and improves fruit and seed production. The objective of this study to evaluate the efficacy of nineteen Trichoderma isolates to produce Indole Acetic Acid (IAA), Hydrogen Cyanide (HCN) and Ammonia (NH3). In present study, nineteen isolates of Trichoderma spp. were tested for their ability to produce IAA, HCN and NH3 on Czapek-Dox medium, Malt Extract Agar medium and 1% peptone water. Among the 19 isolates used, isolates 101 and 102 showed significantly higher percentage of IAA production whereas isolate Number- 22 and 36 were weakest IAA producer after 17th days of incubation. 3 isolates were moderate producers of HCN i.e., isolate number- 13, 59 and 73. And 6 isolates (isolate number-22, 62, 72, 93, 95 and 102) were detected as weak producers of HCN. NH3 production was detected in 100% isolates, out of them isolate number- 4, 13, 22, 23, 37, 59, 62, 70, 101 and 102 were strong producers while isolate number- 24, 36, 72, 73, 89, 93, 95, 97 and 99 were weak producers. The study confined that all nineteen isolates that were used, showed the good production of IAA, HCN, NH3. Hence these can be applied in the field as Plant Growth Promoting Fungi (PGPF).