Synthesis of Silver Nanoparticles: Characterisation and Antimicrobial Activity of Synergistic Herbal Formulation Derived from Cassia fistula and Manilkara zapota Plants

Authors

Archana Hari, Research Scholar, Vijaya Geetha Bose, Assistant Professor
Department of Biotechnology, Rajalakshmi Engineering College (Autonomous), Affiliated to Anna University, Thandalam, Chennai, Tamil Nadu, India.

Abstract

The synergism of plants in herbal medicine has been more effective than the individual ones. Synthesis of silver nanoparticles was taken from the synergistic methanol extract of Cassia fistula flowers and leaves and Manilkara zapota leaves. This extract of plants was assessed for its major phytoconstituents and antimicrobial activity. Here in this investigation methanol concentrate of flowers and leaves of Cassia fistula and leaves of Manilkara zapota was used to synthesise silver nanoparticles. The silver nanoparticles were then characterised by the UV – visible spectrophotometer, FourierTrans form Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). In the UV- visible spectroscopy, maximum absorption was obtained at 425 nm. In SEM analysis, the dimensions of the particle were irregular and ranged from 100nm – 150nm. In FTIR, different stretch of bonds was shown at 3332.16,2114.20,1635.70 and 637.27. The antibacterial assay of plant silver nanoparticles in case of Staphylococcus aureus, showed its maximum zone of inhibition at 17mm. Thus,the easily available ecofriendly plants and the synergistic consequence of the plants with the advanced nanoparticle technique will show revolution in health care and to cure various health issues .