INVITRO ANTI-CANCER STUDY OF HERBAL EXTRACT ON COLON CARCINOMA CELLS

Authors

Deeparani Urolagin, Professor
Depatment of Pharmacology,ABIPER,Bangalore,Karnatatka.

Hani Umme, Assistant professor
King khalid University,Abha,Saudi arabia.

Archana SP, Assistant proessor
KLE college of Pharmacy,Belgaum. 

Abstract

Introduction: Plant products are important and attractive in field of scientific investigation are considered a rich source of pharmacologically active molecules and for new drug development. The current research aimed to assess anticancer effect of herbal extract on colo-205 cancer cell lines. . Here, we investigated anti-cancer effects of three herbal drug extracts-Ixora coccinea family Rubiaceae flower and Piper longum family Piperaceae, vitis vinifere family vitiaceae-Grape seed (GSE) root extract to treat against colon cancer . Method: Several qualitative phytochemical tests were performed on Ixora coccinea,Piper longum and vitis vinifere by using standard phytochemical procedures to assessment of cytotoxic MTT assay using standard biochemical methods in Colo-205 cells .With supplement of 1% penicillin,of 10% inactivated Fetal Bovine Serum,cells were cultured in DMEM , using 5% CO2 at humidified atmosphere(37oC). Results:The percentage inhibition(IC50 value) of Piper longum (0–640 µg/ml),vitis vinifera (0–640 µg/ml) and Ixora coccinea (0–640 µg/ml) treatment of cells were showed following result, 57% (636 µg/ml), 52% (366µg/ml) and 58% (244µg/ml) growth inhibition respectively. Decreased cell proliferation in time-dependent manner was observed.