Induction and resuscitation of viable but nonculturable bacteria from different taxonomic groups

Authors

Yury D. Pakhomov, FSBSI Mechnikov Research Institute for Vaccines and Sera, Maliy Kazenniy per, 5a, Moscow, Russia.

Larisa.P. Blinkova, Assistant Manager
FSBSI Mechnikov Research Institute for Vaccines and Sera, Maliy Kazenniy per, 5a, Moscow, Russia.

Abdullaeva, FSBEI Moscow State University of food production, Volokolamskoye Shosse, 11, Moscow, Russia.

Rumya.K. Valitova, FSBSI Mechnikov Research Institute for Vaccines and Sera, Maliy Kazenniy per, 5a, Moscow, Russia,
FSBEI Moscow State University of food production, Volokolamskoye Shosse, 11, Moscow, Russia.

Tatiana A. Karachina, FSBEI Moscow State University of food production, Volokolamskoye Shosse, 11, Moscow, Russia.

Abstract

We obtained nonculturable cells of Escherichia coli M17, Salmonella Typhimurium 79, and Staphylococcus aureus FDA 209P under prolonged osmotic and trophic stresses. It was noted, that even in populations incubated under microaerophilic conditions the percentage of nonculturable cells exceeded 99% during long-term observation. The data gathered in the experiments showed that the combination of incubation of cells in normal saline and inoculation on a solid medium with a diluted nutrient base is necessary to get out of the nonculturable state. This combination of factors with a reduced level of nutrients provides gentle conditions for the growth of microorganisms that have been starving for a long time in conditions of increased osmotic pressure.