In-Vitro Evaluation of Anti-Cancer Activity of Actinidia Deliciosa And Brassica Juncea on Human Lung Carcinoma Cells (A549)

Authors

V. Manivasagan, S. Subratha, V. Daricca, K. Saranya & N. G. Ramesh Babu
Department of Biotechnology, Adhiyamaan College of Engineering, Hosur-635130 Tamilnadu, India.

Abstract

Actinidia fruits have been captivate, mainly due to the highly described health benefits. Brassica is the economically important genus. Glucosinolates and sterols are the chief chemical constituents of Brassica have chemoprotective effect in different types of cancer. Methanolic extract of Brassica juncea and Actinidia deliciosa, phytochemical analysis, subjected to DPPH method, qualitative analysis using HPLC, antiproliferation activity using MTT assay on A549 cell line, study of apoptosis by flow cytometry method using A549 cells and evaluation of cell lysis by Hemolysis assay were carried out. In extraction process the sample yield was 444 mg of A. deliciosa and 314 mg of B. juncea. The samples revealed the existence of steroids, alkaloids, protein, flavonoids, terpenoids and carbohydrates. B. juncea alone shows the existence of phenol and tannins. A. deliciosa was obtained to be 28. 26 µg/ mL of IC50 value antioxidant activity higher than 95 µg/ mL IC50 value of B. juncea. Quercetin taken as standard for antioxidant activity. The purity was checked using HPLC it showed 88 % B. juncea is higher than 79 % A. deliciosa. The cytotoxity of A. deliciosa shows better action contrary to A549 cells. Thus, A. deliciosa was taken for apoptosis study. Doxorubicin was taken as standard drug which treats cancer in lung, bladder, ovarian and so on. The viability of cells at 80 µg/ mL was 75. 87 % whereas at 160 µg/ mL was 66. 31 % in apoptosis using flow cytometry. The attained data has negligible haemolysis and samples can be taken for pharmacological studies. In future studies the cell viability against cancer can be reduced by further purification. Therefore, our findings will ameliorate the drug development.