Evaluation of genetic diversity of domestic and wild pistachio genotypes in IRAN (Khorasan Razavi) using ISSR markers

Authors

Esmail Mirahmadi
Ms.d. Department of Plant Production, Faculty of Agriculture, University of Torbat Heydarieh, Torbat Heydarieh, Iran.

Iman Yousefi Javan, Assistant Professor
Department of Plant Production, Faculty of Agriculture, University of Torbat Heydarieh, Torbat Heydarieh, Iran.

Ahmad Ahmadian, Assistant Professor
Department of Plant Production, Faculty of Agriculture, University of Torbat Heydarieh, Torbat Heydarieh, Iran.

Masoud Alipanah, Associate Professor
Department of Plant Production, Faculty of Agriculture, University of Torbat Heydarieh, Torbat Heydarieh, Iran.

Abstract

Pistacia vera L. is one of the most important horticultural products of Iran which has a large share of non-oil export earnings. Optimal use of any product requires a rational understanding of its genetic diversity status in nature. Genetic sources of pistachio, identification and identification of vegetative and reproductive characteristics of cultivars and phenotypes of this plant are among the most important pistachio breeding measures. In this study, genetic diversity of 20 pistachio genotypes collected from Khorasan Razavi pistachio, Pistacia atlantica and wild pistachio (Pistacia vera) genotypes were evaluated using 20 ISSR primers. that yielding 74 scaffold bands in total. Among these, 35 bands (47.30%) showed polymorphism. Genotypes were divided into 4 groups based on cluster analysis by UPGMA method and Jaccard similarity coefficient. According to the results, ISSR was recognized as a repeatable method for identifying genotypes in pistachio genus. Ultimately, this will save considerable time and expedite corrective programs.