Development of In Vitro Propagation Protocol for Origanum vulgare L. (Lamiaceae) – an Important Culinary and Medicinal Herb

Authors

Sayyed Ashfaqullah, Saumiya Agnihotri, Preeti Dhobhal – Research Scholars
Plant Tissue Culture Laboratory, Department of Botany, DSB Campus, Kumaun University Nainital, Uttarakhand, 263001.

Yasmeen Akhter, B.Lib
Department of Library sciences, University of Jammu, Jammu and Kashmir, 180006. 

Sushma Tamta, Associate professor
Plant Tissue Culture Laboratory, Department of Botany, DSB Campus, Kumaun University Nainital, Uttarakhand, 263001. 

Abstract

Origanum vulgare L. is a high value culinary and medicinal plant that has an ancient history of being used in the treatment of many health ailments. Because of high biologically active compounds encompassing the plant in vitro production can be an alternative to its collection from nature and will also aid in the conservation of the species. The widely accepted Murashige and Skoog nutrient medium was selected for in vitro propagation of the plant. Plant Growth Regulators like 6-Benzyladenine (BA), ἀ-Naphthylacetic acid (NAA), Indole-3-acetic acid (IAA) Indole-3-butyric acid (IBA) and Kinetin (Kn.) were used in the current study. Nodal and apical segments were taken as initial explants. The shoot formation occurred on MS basal medium (PGR free medium) while shoots multiplied best when MS medium was supplemented with BA along with NAA at concentration of 2.0µM/l and 0.30µM/l respectively. After successful multiplication IAA at 6µM/l of concentration resulted to be the best hormone for inducing roots. Eighty percent survival rate was recorded after 45 days of acclimatization.