Assessment of Histoarchitecture Antioxidant and Anti-Cholinesterase Activity of Methanolic Extract from Nigella Sativa Linn.

Authors

S. Sudha, Ph.D Scholar
Department of Biotechnology, Srimad Andavan Arts and Science College (Autonomous), (Affiliated to Bharathidasan University), Tiruchirappalli – 600 005, Tamilnadu, India. 

C. Janani, B. Chitra and S. Arif Nisha, Assistant Professors
Department of Biotechnology, Srimad Andavan Arts and Science College (Autonomous), (Affiliated to Bharathidasan University), Tiruchirappalli – 600 005, Tamilnadu, India. 

Abstract

Medicinal plants synthesize numerous active chemical compounds for functions, including the most significant interest of pharmacological preparations. Kalojeera is also known as Nigella sativa Linn. (Ranunculaceae) is a natural flavouring and food preservative that is used to treat a variety of diseases such as inflammation, asthma, and bronchitis.The bioactive compounds were treated with nervous system disease, epilepsy, pain, memory impairment and neurotoxicity pain. Recent research on the neuroprotective efficacy of N. Sativa is still in its infancy.As a result, the current research aims to test Nigella sativa’s antiability. Alzheimer’s GC-MS analysis was used to classify the extract’s phytochemical composition. Antioxidant and anticholinesterase activity were also examined on the extracts. The current findings suggest that N. Sativa seeds have significant antioxidant and anticholinesterase properties (P<0.05), with AChE inhibitory activity of 91±84. 50% at a 500 μg /ml concentration of N. Sativa.The antioxidant assay results suggest that the extract showed noticeable hydrogen peroxide scavenging activity of 98 ±84.50%, DPPH radical scavenging activity of 99±67. 50% and Reducing power potential demonstrated that the highest effect of 99±84.50 was with standard antioxidant ascorbic acid.Furthermore, the antioxidant and anticholinesterase potential were found to have a positive correlation(p < 0.05). Linoleic acid was found in the methanolic extract of N. Sativa by GC-MS analysis, which could contribute to the above-mentioned activities. The protection of N. Sativa extract was calculated using the Danio rario (zebrafish) liver function test and histoarchitecture from Brain, gill and Liver results show it does not affect liver function.The tissues of the brain, gills, and liver are all natural. Cholinesterase inhibitory activity, antioxidant activity, liver function test, and histoarchitecture were all excellent in the extract. Hence N. Sativa could be a potential lead and therapeutic compound for Alzheimer's disease.