Ascertaining Wound Healing Properties of Typha angustata L. Inflorescence

Authors

Shraddha Saha, Dhruti Patel, Sumitra Chakraborty, Bhavin Vyas, Shrikant Joshi & Meonis Pithawala
C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Maliba Campus, Bardoli, Surat 394350, Gujarat, India.

Abstract

The present study was initiated with an objective to determine the wound healing potential of aqueous crude extract prepared from the inflorescence of Typha angustata L, commonly known as cattail. The extract was subjected to LC-MS metabolite profiling and cytotoxicity analysis by MTT assay. Wound healing efficacy of crude extract was determined by haemostatic and angiogenic potentials. Chick Chorioallantoic Membrane (CAM) Assay was used as a parameter to check angiogenesis. The LC-MS profiling confirmed the presence of phenolics, flavonoids, triterpenes, alkaloids, carbohydrate, glycosides etc. in the inflorescence crude extract. Additionally, cytotoxicity study results revealed that the IC50 value of T. angustata L. inflorescence after 24 hours of treatment was found to be in non toxic range. The haemostatic activity assessed by determining Clotting Time (CT) and Prothrombin Time (PT) revealed that blood samples treated with crude extract caused significant (p<0.05) reduction of CT and PT values at tested concentrations as compared to control. Angiogenesis ability of an extract was assessed by selected angiogenesis parameters viz., percentage increase in extremities, nodes, junctions etc. in the experimental treated groups and control group. SPSS 16 software and one way ANOVA were used for statistical analysis. The results of the study revealed that all selected angiogenesis parameters showed significant increase (p<0.05) in concentration and time dependent manner as compared to control.