Alteration of Media Enables Efficient In-vitro Regeneration in Antamool (Tylophora indica): A Threatened Medicinal Plant

Authors

Pooja Devi, Ranjita Singh, Kumud Kant Awasthi, Garima Awasthi
Faculty of Basic & Applied Sciences, Vivekananda Global University, Jaipur India.

Rajesh Kumar
Chaudhary Devi Lal University, Sirsa Haryana India.

Mithilesh Kumar
School of Life Sciences, Jaipur National University, Jaipur India.

Tanvi SinghUniversity of Delhi, India.

Abstract

ylophora indica commonly known as antamool is a threatened medicinal plant. Native species of this plant is decreasing very rapidly due to complete lack of organized cultivation and over- exploitation. Therefore, an efficient protocol for large-scale T. indica multiplication needs to be established. Current study on T. indica is conducted to develop a beneficial, consistent & feasible method for mass multiplication in in-vitro controlled conditions. Murashige and Skoog medium was used as a basal medium to culture T. indica and to studyorganogenesis and somatic embryogenesis. Further, the presence of secondary metabolite was detected by Thin Layer Chromatography technique. Callus was developed on Murashige and Skoog medium augmented with varying concentrations of 6- benzylaminopurine (BAP), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) with 3% sucrose and 50 mg/l casein hydrolysate. Highest rate of organogenic and embryogenic calli was observed at 0.5 mg/l NAA +1.0 mg/l BAP and 1.0 mg/l 2,4-D + 0.5mg/l BAP. Differentiation of shoot buds was best achieved from the callus surface after transferring into shooting media. The highest rate (100%) of shoot bud initiation was observed on MS medium containing 0.5mg/l NAA +1.0mg/l BAP and 1.0mg/l NAA +1.5mg/l. BAP.Similarly, somatic embryos were induced on Murashige and Skoog medium supplemented with 1.0mg/l 2,4-D and 0.1% potassium nitrate.The present research provides evidence of significant organogenic and embryogenic potency of T.indica as its exhibits callus formation and shoot buds formation at higher rate. The above method will allow this medicinally important plant to be produced on a large scale for the vegetation, nurseries and for the pharmaceutical corporations.